Coding
LexA DBD

Part:BBa_K807001:Design

Designed by: Ian Roney   Group: iGEM12_uOttawa_CA   (2012-09-28)

Yeast Codon Optimized LexA DNA Binding Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 47


Design Notes

Had to take the LexA CDS and select the DNA binding domain only, with no stop codon.

Source

Sequence was derived from the E.coli LexA repressor.

References